Intrusion detection methods based on Channel State Information(CSI) are vulnerable to environment layout and noise interference, resulting in low detection rate. To solve this problem, an indoor intrusion detection method based on the algorithm of Direction-Of-Arrival(DOA) estimation for single snapshot was proposed. Firstly, the CSI data received by the antenna array was mathematically decomposed by combining the feature of spatial selective fading of the wireless signals, and the unknown DOA estimation problem was transformed into an over-complete representation problem. Secondly, the sparsity of the sparse signal was constrained by l1 norm, and the accurate DOA information was obtained by solving the sparse regularized optimization problem, so as to provide the reliable feature parameters for the final detection results at data level. Finally, the Indoor Safety Index Number(ISIN) was evaluated according to the DOA changes before and after the moments, and then indoor intrusion detection was realized. In the experiment, the method was verified by real indoor scenes and compared with traditional data preprocessing methods of principal component analysis and discrete wavelet transform. Experimental results show that the proposed method can accurately detect the occurrence of intrusion in different complex indoor environments, with an average detection rate of more than 98%, and has better performance in robustness compared to comparison algorithms.